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Analysis of surface markers and multipotency of stromal cells in biosheets (A) Representative flow cytometry profiles of cells isolated from 2w-biosheets and identification of a population of quadruple-positive cells (P7). (B) The ratio of quadruple-positive cells (P7) to stromal cells (P5) in the biosheets and fascia. (C-E) Representative images of alkaline phosphatase (ALP) staining and Alizarin red staining (C), oil red staining (D), Toluidine blue (TB) staining and Safranine O (SO) staining (E) of stromal cells isolated from biosheets and subjected to osteogenic, adipogenic and <t>chondrogenic</t> differentiation. (F-H) Expression levels of osteogenic (F), adipogenic (G), and chondrogenic (H) marker genes. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Analysis of surface markers and multipotency of stromal cells in biosheets (A) Representative flow cytometry profiles of cells isolated from 2w-biosheets and identification of a population of quadruple-positive cells (P7). (B) The ratio of quadruple-positive cells (P7) to stromal cells (P5) in the biosheets and fascia. (C-E) Representative images of alkaline phosphatase (ALP) staining and Alizarin red staining (C), oil red staining (D), Toluidine blue (TB) staining and Safranine O (SO) staining (E) of stromal cells isolated from biosheets and subjected to osteogenic, adipogenic and <t>chondrogenic</t> differentiation. (F-H) Expression levels of osteogenic (F), adipogenic (G), and chondrogenic (H) marker genes. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Analysis of surface markers and multipotency of stromal cells in biosheets (A) Representative flow cytometry profiles of cells isolated from 2w-biosheets and identification of a population of quadruple-positive cells (P7). (B) The ratio of quadruple-positive cells (P7) to stromal cells (P5) in the biosheets and fascia. (C-E) Representative images of alkaline phosphatase (ALP) staining and Alizarin red staining (C), oil red staining (D), Toluidine blue (TB) staining and Safranine O (SO) staining (E) of stromal cells isolated from biosheets and subjected to osteogenic, adipogenic and <t>chondrogenic</t> differentiation. (F-H) Expression levels of osteogenic (F), adipogenic (G), and chondrogenic (H) marker genes. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Analysis of surface markers and multipotency of stromal cells in biosheets (A) Representative flow cytometry profiles of cells isolated from 2w-biosheets and identification of a population of quadruple-positive cells (P7). (B) The ratio of quadruple-positive cells (P7) to stromal cells (P5) in the biosheets and fascia. (C-E) Representative images of alkaline phosphatase (ALP) staining and Alizarin red staining (C), oil red staining (D), Toluidine blue (TB) staining and Safranine O (SO) staining (E) of stromal cells isolated from biosheets and subjected to osteogenic, adipogenic and chondrogenic differentiation. (F-H) Expression levels of osteogenic (F), adipogenic (G), and chondrogenic (H) marker genes. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: Hepatocyte growth factor in biosheets promotes autonomous regeneration of cutaneous tissue after transplantation onto a full-thickness skin defect

doi: 10.1016/j.mtbio.2026.102969

Figure Lengend Snippet: Analysis of surface markers and multipotency of stromal cells in biosheets (A) Representative flow cytometry profiles of cells isolated from 2w-biosheets and identification of a population of quadruple-positive cells (P7). (B) The ratio of quadruple-positive cells (P7) to stromal cells (P5) in the biosheets and fascia. (C-E) Representative images of alkaline phosphatase (ALP) staining and Alizarin red staining (C), oil red staining (D), Toluidine blue (TB) staining and Safranine O (SO) staining (E) of stromal cells isolated from biosheets and subjected to osteogenic, adipogenic and chondrogenic differentiation. (F-H) Expression levels of osteogenic (F), adipogenic (G), and chondrogenic (H) marker genes. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The cells formed pellets after 3 days of culture, and the medium was replaced with MSC Chondrogenic Differentiation Medium (C-28012, PromoCell).

Techniques: Flow Cytometry, Isolation, Staining, Expressing, Marker